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Accordingly, as used herein, the terms “nucleotide sequence to be transcribed” or “target nucleotide sequence” are intended to include both the nucleotide sequence which is transcribed into mRNA and an operatively linked upstream minimal promoter sequence? A mutated Tet repressor can be created and selected, for example as follows: a nucleic acid (eg, DNA) encoding a wild-type Tet repressor is subjected to random mutagenesis and the resultant mutated nucleic acids are incorporated into an expression vector and introduced into a host cell for screening!
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